WG-TIRF (Fig.1) is an optional unit that allows easy addition of the TIRF(Total Reflection Fluorescence Microscopy) function to Commercially available microscopes.
For an explanation of the TIRF and the difference between WG-TIRF and the major manufacturer's TIRF, plase refer to Technology page. The following is a brief summary of the features of our WG-TIRF for a major manufacturer's TIRF.
Major manufacturer's (Objective lens type)
Very expensive（Several hudred thousand dollers）
It is suitable for high magnification observation (x60 or more).
Low magnification (wide field of view) obseration is impossible.
Adjustment is severe, but if successful, very high resolution images can be obtained. -→ High-end use
WG-TIRF (prism type)
Low price（Basic SET $20,000-，1/10 or less of major manufacturers' products）
It is suitable for low magnification (wide field of view) observation.
It is darken in high mabnification.
Images with higher contrast than usual can be acquired for routine observation. -→ Daily use
Manufacturer's TIRF is a product that is positioned between laser confocal microscopes and super-resolution microscopes, and has not become so popular as true high-end users are shifting to super-resolution microscopes. We developed WG-TIRF for whitch the observation technology, known as TIRF, used some exparts can be used widely by user who use microscope as routine observation. Even research instruments must first be inexpensive to disseminate. In addition, it is require to have a good balance between price and performance and to meet the needs of researchers.
This section briefly introduces the configuration, use, functions, and observation images of WG-TIRF devices.
It mainly consists of two units: the main body (right in Fig. 1) and the power supply BOX(left in Fig. 1).
How to use
Place it on the stage of the microscope as shown in Fig.2.
Fig.2 Installation Image on microscope
Dark field (scattered light) observation and fluorescence observation by total reflection illumination are possible.
Two total reflection optics allow fluorescence observation of double stained samples.
By replacing the attachment of the sample table, it can be used for both inverted and upright microscope.